Cover Letter: Run 112/41

Dear UK NEQAS ICC & ISH Participant,

  • Run 111 / Run 40 reports are available to download.
  • Run 112 ICC / Run 41 ISH slides will be despatched between the 26th – 30th October 2015.

Click on ‘Participant login’ to retrieve reports and instructions for the new assessment

Closing Date for RUN 112 (ICC) / 41 (ISH): 27th November 2015*
 *Slides post-marked after this date may not be assessed. Late receipts are logged and reviewed by the scheme.

  • Confirm receipt of your slides via:
  • Allow at least 1 week (2 weeks for overseas labs) after the last despatch date for your slides to arrive. If you do not receive your slides, please contact or Tel: +44 2075548679.

Important Changes to the Distributed EQA Slides

Distributed EQA slides now consist of ‘same slide NEQAS + in-house’ control slides. Please cut your appropriate in-house control material alongside the NEQAS slide.

  • Separate in-house controls are still required for the Cytology Module
  • All slides except the Cytology module will arrive unbaked
  • Please cut your in-house controls onto the NEQAS slide below the red line, drain thoroughly
  • Then bake the slide. Recommended baking times are either 37°C overnight OR 55-60°C for 1 hour, and then carry out your usual staining procedure.

Module Specific Announcements

See also module specific instruction sheets which are available by clicking ‘instructions’ in the participant login area

  • Breast HER2 ISH: Cut and place a single ‘example’ from a clinical case or control onto the distributed NEQAS slide. See online module instruction sheet. A composite control is NOT required.
  • ALK IHC for NSCLC: Reports are now available in participants’ login area: Instructions and methodology data entry are available by clicking on ‘Ex.URL’ link once logged into the web data entry system.
  • Breast Hormone Receptor: Antibody Requested = Progesterone receptor (PR). If PR is not on your repertoire than ER should be carried out
  • Cytology: The Gold for this run is S100 only, do not submit Melan A or HMB45 or these will be returned.
    In-house controls should still be sent as a separate slide. We are not using the Patient/Control slides for this module. If you wish to change your sample (cytospin or cell block) then please contact the scheme.
  • Secondary Antibodies Run 112:
    Gen C: Ber-EP4; Lymphoma N: CD30 ; Neuropath J: cytokeratin;
    Cytology T: CK5/6; GIST Vb: DOG-1; HNPCC: MLH1 & PMS2
  • Antibody not stocked?: Please e-mail for the alternative antibody that you require:


  1. Forgotten Your Login Details?: Please use the ‘Email Reminder’ link on to retrieve your details. UK NEQAS ICC & ISH will not give out logon details to any laboratory personnel not registered on the scheme’s database.
  2. Labelling Of Slides and Requests For Extra Slides
    • Remove your machine generated/manually written labels from the EQA slides
    • Make sure separate in-house slides are clearly labelled with your lab and run number
    • Return slides in the correct , colour coded, pre-labelled boxes, sealed and labelled for each module.
    • The last date for requesting extra slides is 7 days prior to the closing date
    • A valid reason for requesting extra slides is required. Extra attempts are not permitted.
    • Between assessments we are happy to provide extra sections for trouble shooting or validation.
  3. Completion of Online Data Sheets is Mandatory
    • We assess not only your slides but require your methods/protocols to give the best feedback possible. Remember you must enter data for both antibodies before pressing Complete; prior to this use the Save button until both sets of data have been entered.
  4. Web Data Entry
    • Slide Instructions are available in the Methods/Protocols Section
    • ‘Help’ files are available online once you log into your web data entry e.g.:
  5. Reassessments/Appeals
    • Are only accepted for the latest assessed slides
    • Prior to requesting a Reassessment/Appeal, participants you are expected to have received your slides back and looked at these along with your report and the images on the website
    • Download a re-assessment form from:
    • Only enter the details for the slides you wish to be reassessed; if you are sending in slides for comparison only; e.g. in-house controls please mention this in the section at the bottom of the reassessment form.
    • Please do not use any previous versions of these forms, or you may be asked to resubmit.
  6. Diagnostic testing (UK labs only)
    • If your laboratory is NOT currently diagnostically testing for any of the UK NEQAS ICC & ISH modules, then please inform us immediately, otherwise this can impact on your poor performance status
  7. Non submissions – reasons entered in the online data
    • If you are not participating for a particular module or run please ensure you select the correct reason from the drop down menu in the data entry; e.g. antibody not stocked, outsourcing, withdrawn, even if you have contacted the scheme or a member of the UK NEQAS ICC & ISH team, or returned an online survey etc.

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